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10x translation buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 10x translation buffer
    10x Translation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10x+translation+buffer/HEPES/pm41465210-306-15-57
    Average 99 stars, based on 1 article reviews
    10x translation buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Solvent:

    Article Title: Characterization of Bacillus velezensis EV17 and K-3618 and Their Polyketide Antibiotic Oxydifficidin, an Inhibitor of Prokaryotic Translation with Low Cytotoxicity.
    Article Snippet: .. The reaction was carried out in 10 μL, including 5 μL HEK293T extract, 1 μL 10X translation buffer (20 mM Hepes-KOH pH 7.6, 1 mM DTT, 0.5 mM spermidine-HCl, 0.8 mM Mg(OAc)2, 8 mM creatine phosphate, 1 mM ATP, 0.2 mM GTP, 120 mM KOAc and 25 μM of each amino acid), 2U of RiboLock RNase inhibitor (Thermo Scientific), 0.5 mM d-luciferin (Promega), 1 μL of either antibiotic solution or solvent (water), and 50 ng mRNA (the latter was added to 1 μL of the mixture solution after preliminary incubation of the reaction mixture with the antibiotic for 5 min at 30 ◦C.). .. After adding the mRNA, the mixtures were transferred to a pre-heated white FB/NB 384 well plate (Grenier no. 781904) and incubated in the VICTOR X5 Multilabel Plate Reader (PerkinElmer, Waltham, MA, USA) at 30 ◦C with continuous measurement of luciferase activity.

    Incubation:

    Article Title: Characterization of Bacillus velezensis EV17 and K-3618 and Their Polyketide Antibiotic Oxydifficidin, an Inhibitor of Prokaryotic Translation with Low Cytotoxicity.
    Article Snippet: .. The reaction was carried out in 10 μL, including 5 μL HEK293T extract, 1 μL 10X translation buffer (20 mM Hepes-KOH pH 7.6, 1 mM DTT, 0.5 mM spermidine-HCl, 0.8 mM Mg(OAc)2, 8 mM creatine phosphate, 1 mM ATP, 0.2 mM GTP, 120 mM KOAc and 25 μM of each amino acid), 2U of RiboLock RNase inhibitor (Thermo Scientific), 0.5 mM d-luciferin (Promega), 1 μL of either antibiotic solution or solvent (water), and 50 ng mRNA (the latter was added to 1 μL of the mixture solution after preliminary incubation of the reaction mixture with the antibiotic for 5 min at 30 ◦C.). .. After adding the mRNA, the mixtures were transferred to a pre-heated white FB/NB 384 well plate (Grenier no. 781904) and incubated in the VICTOR X5 Multilabel Plate Reader (PerkinElmer, Waltham, MA, USA) at 30 ◦C with continuous measurement of luciferase activity.



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    10x translation buffer  (Thermo Fisher)
    99
    Thermo Fisher 10x translation buffer
    10x Translation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10x+translation+buffer/HEPES/pm41465210-306-15-57
    Average 99 stars, based on 1 article reviews
    10x translation buffer - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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